CD8 antibody (PE)

Catalog No. ABIN118134

This anti-CD8 antibody is a Rat Monoclonal antibody detecting CD8 in FACS. Suitable for Mouse.

Quick Overview for CD8 antibody (PE) (ABIN118134)

Target: CD8

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: KT15

Product Details anti-CD8 Antibody (PE)

Specificity: This antibody reacts to Ly2.1 and Ly2.2 cells. Reacts with the CD8 alpha chain (non polymorphic). Weakly cytotoxic.

Immunogen: T cell clone, C6. Spleen cells from a Spraque Dawley rat immunised with the C6 T cell clone were fused with cells of the NSO mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled water: SM011R with 1.0 mL SM011RT with 0.25 mL SM011RX with 5.0 ml

Buffer: PBS containing 0.09 % Sodium Azide as presrevative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 12525

NCBI Accession: NP_001074579

UniProt: P01731